ISO 21446:2019 Preview

Infant formula and adult nutritionals -- Determination of trans and total (cis + trans) vitamin K1 content -- Normal phase HPLC

This document specifies a method for the quantitative determination of trans and total (cis + trans) vitamin K1 in infant, pediatric and adult nutritionals using normal phase (NP) high-performance liquid chromatography (HPLC) with post-column reduction and fluorescence detection. The method demonstrated good linearity over a standard range of ~2 μg/l to 80 μg/l trans vitamin K1, and the limit of quantification (LOQ) was estimated to be 0,4 μg/l for standards and 0,09 μg/100 g ready to feed (RTF) for samples assuming 4 grams of sample are diluted to 10 ml.


General information

  • Status :  Published
    Publication date : 2019-01
  • Edition : 1
    Number of pages : 20
  • :
    ISO/TC 34
    Food products
  • 67.050
    General methods of tests and analysis for food products

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